RESUMO
Stenotrophomonas maltophilia, an aerobic, non-fermenting, Gram-negative bacterium, is an emerging nosocomial pathogen. It is considered to be a low-grade pathogen, and infections due to S. maltophilia are uncommon. However, in the recent past, S. maltophilia infections have been on the rise, particularly in patients who are either immunocompromised, aged or on long-term antibiotic therapy. Endocarditis due to S. maltophilia is extremely rare. This is a report of two patients with S. maltophilia endocarditis who were diagnosed by 16S rRNA gene sequencing.
Assuntos
Endocardite , Infecções por Bactérias Gram-Negativas , Stenotrophomonas maltophilia , Idoso , Antibacterianos/uso terapêutico , Endocardite/complicações , Endocardite/diagnóstico , Endocardite/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Humanos , RNA Ribossômico 16S/genética , Stenotrophomonas maltophilia/genéticaRESUMO
Q fever is a zoonotic disease caused by the obligate intracellular bacterium Coxiella burnetii. Infective endocarditis is the most common form of chronic Q fever. Diagnosis of Q fever is difficult, as there are no pathognomonic symptoms. Methods of isolation of the organism in culture are tedious, hence serological and molecular techniques remain the mainstay of diagnosis. We report two cases of Q fever endocarditis diagnosed by IFA and real-time PCR.
Assuntos
Coxiella burnetii , Endocardite Bacteriana , Endocardite , Febre Q , Coxiella burnetii/genética , Endocardite/diagnóstico , Endocardite/microbiologia , Endocardite Bacteriana/diagnóstico , Endocardite Bacteriana/microbiologia , Humanos , Índia , Febre Q/diagnóstico , Febre Q/microbiologiaRESUMO
INTRODUCTION: Oral health is suspected to be linked to heart disease since species of bacteria that cause periodontitis and dental caries have been found in the atherosclerotic plaque in arteries in the heart. OBJECTIVES: The aim of this study was to characterize the oral microbiome in patients with coronary artery disease (CAD) and in a patient with dental caries (DC) without any clinical symptoms of CAD. METHODS: DNA was extracted from the oral swabs collected from the patients and sequencing was performed by next generation sequencing method using Illumina (MiSeq) platform. The resulting sequencing data set was analysed using QIIME. RESULTS: A total of 31 phyla were found in all the samples. The predominant phylum found in both CAD and DC was Firmicutes (46.09% & 38.98%), Proteobacteria (17.73% & 9.79%), Fusobacteria (13.44% & 17.95%), Bacteroidetes (11.82% & 22.73%), Actinobacteria (8.33% & 7.71%) and TM7 (2.25% & 2.71%). We found a similarity in the bacterial diversity in the two groups of patients. CONCLUSION: A comparison of the oral microbiome in patients with CAD and DC shows a similarity in the composition of the oral microbiota with variations in the proportion of a few genera.
